Journal
AMB EXPRESS
Volume 8, Issue -, Pages 1-11Publisher
SPRINGEROPEN
DOI: 10.1186/s13568-018-0565-8
Keywords
Grifola frondosa; Crop straw; Simplex-lattice design; Yield and growth cycle; High-yielding formula; Biological efficiency
Categories
Funding
- Special Fund for Agro-scientific Research in the Public Interest [201503137]
- National Key Research and Development Program of China [2017YFD0601002]
- program of creation and utilization of germplasm of mushroom crop of 111 project [D17014]
- National Basic Research Program of China [2014CB138305]
- University S AMP
- T Innovation Platform of Jilin Province for Economic Fungi [2014B-1]
- National Natural Science Foundation of China [31471926]
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Grifola frondosa is an economically important edible and medicinal mushroom usually produced on substrate consisting of sawdust supplemented with wheat bran. Cultivation of G. frondosa on crop straw (corn cob, corn straw, rice straw, and soybean straw) as a substrate was optimized by using the D-optimum method of the simplex-lattice design, and the alternative of crop straw as a substitute for sawdust in the substrate composition was determined by the optimized model. The results showed that there was a significant positive correlation existing between the yield and corn cob. The growth cycle was negatively correlated with sawdust, corn cob and soybean straw, with sawdust significantly shortening the growth cycle of G. frondosa. The optimized high-yielding formula included 73.125% corn cob, 1.875% rice straw, 23% wheat bran and 2% light calcium carbonate (CaCO3) (C/N = 48.40). The average yield of the first flush was 134.72 +/- 4.24 g/bag, which was increased by 39.97% compared with the control formula. The biological efficiency (BE) was 44.91 +/- 1.41%, which was increased by 38.53% compared with the control. Based on the results of this study, corn cob can replace sawdust as one of the main cultivation substrates of G. frondosa.
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