Journal
ADVANCED SCIENCE
Volume 5, Issue 4, Pages -Publisher
WILEY
DOI: 10.1002/advs.201700611
Keywords
CRISPR/Cas9 system; exosomes; hybrid nanoparticles; targeted delivery
Categories
Funding
- National Natural Science Foundation of China [81570803]
- Guangzhou Foundation for Science and Technology Planning Project, China [201704030083]
- Science and Technology Planning Project of Guangdong Province, China [2017A050501013]
- Fundamental Research Funds for the Central Universities [17ykjc21]
- Oral Medicine Research Foundation of Guangdong Provincial Key Laboratory [KF2016120104]
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Targeted delivery of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system to the receptor cells is essential for in vivo gene editing. Exosomes are intensively studied as a promising targeted drug delivery carrier recently, while limited by their low efficiency in encapsulating of large nucleic acids. Here, a kind of hybrid exosomes with liposomes is developed via simple incubation. Different from the original exosomes, the resultant hybrid nanoparticles efficiently encapsulate large plasmids, including the CRISPR-Cas9 expression vectors, similarly as the liposomes. Moreover, the resultant hybrid nanoparticles can be endocytosed by and express the encapsulated genes in the mesenchymal stem cells (MSCs), which cannot be transfected by the liposome alone. Taken together, the exosome-liposome hybrid nanoparticles can deliver CRISPR-Cas9 system in MSCs and thus be promising in in vivo gene manipulation.
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