Journal
ACS INFECTIOUS DISEASES
Volume 4, Issue 6, Pages 998-1006Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsinfecdis.8b00014
Keywords
discontinuous epitopes; peptide bioprobes; peptide design; microarrays; serodiagnosis; arbovirus; clickable polymer; Zika virus
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Funding
- Regione Lombardia, project READY (Regional Network for developing diagnostic methods in rapid response to emerging epidemics and bioemergencies) [229472]
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Antigen immunoreactivity is often determined by surface regions defined by the 3D juxtapositions of amino acids stretches that are not continuous in the linear sequence. As such, mimicking an antigen immunoreactivity by means of putative linear peptide epitopes for diagnostic purposes is not trivial. Here we present a straightforward and robust method to extend the reach of immune-diagnostic probes design by copresenting peptides belonging to the same antigenic surface. In this case study focused on a computationally predicted Zika virus NS1 protein putative antigenic region, we reached a diagnostic confidence by the oriented and spatially controlled coimmobilization of peptide sequences found adjacent within the protein fold, that cooperatively interacted to provide enhanced immunoreactivity with respect to single linear epitopes. Through our method, we were able to differentiate Zika infected individuals from healthy controls. Remarkably, our strategy fits well with the requirements to build high-throughput screening platforms of linear and mixed peptide libraries, and it could possibly facilitate the rapid identification of conformational immunoreactive regions.
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