Journal
STEM CELL REPORTS
Volume 10, Issue 1, Pages 300-313Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2017.11.001
Keywords
-
Categories
Funding
- National Eye Institute [EY000450, EY000474]
- CENTER FOR INFORMATION TECHNOLOGY [ZIACT000261] Funding Source: NIH RePORTER
- NATIONAL EYE INSTITUTE [Z01EY000450, ZIAEY000450, ZIAEY000546, ZIAEY000474] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [ZICEB000012] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning into a polarized, laminated architecture. In static cultures, organoid development may be hindered by limitations in diffusion of oxygen and nutrients. Herein, we report a bioprocess using rotating-wall vessel (RWV) bioreactors to culture retinal organoids derived from mouse pluripotent stem cells. Organoids in RWV demonstrate enhanced proliferation, with well-defined morphology and improved differentiation of neurons including ganglion cells and S-cone photoreceptors. Furthermore, RWV organoids at day 25 (D25) reveal similar maturation and transcriptome profile as those at D32 in static culture, closely recapitulating spatiotemporal development of postnatal day 6 mouse retina in vivo. Interestingly, however, retinal organoids do not differentiate further under any in vitro condition tested here, suggesting additional requirements for functional maturation. Our studies demonstrate that bioreactors can accelerate and improve organoid growth and differentiation for modeling retinal disease and evaluation of therapies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available