Journal
PEERJ
Volume 6, Issue -, Pages -Publisher
PEERJ INC
DOI: 10.7717/peerj.5111
Keywords
Oocyte; Meiosis; Mycotoxin; Porcine; Mouse
Categories
Funding
- National Basic Research Program of China [2014CB138503]
- National Natural Science Foundation of China [31622055, 31571547]
- Fundamental Research Funds for the Central Universities, China [KYTZ201602, KJYQ201701]
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Background: Atlatoin B1 (AFB1), deoxynivalenol (DON), HT-2, ochratoxin A (OTA), zearalenone (ZEA) are the most common mycotoxins that are found in corn-based animal feed which have multiple toxic effects on animals and humans. Previous studies reported that these mycotoxins impaired mammalian oocyte quality. However, the effective concentrations of mycotoxins to animal oocytes were different. Methods: In this study we aimed to compare the sensitivity of mouse and porcine oocytes to AFB1, DON, HT-2, OTA, and ZEA for mycotoxin research. We adopted the polar body extrusion rate of mouse and porcine oocyte as the standard for the effects of mycotoxins on oocyte maturation. Results and Discussion: Our results showed that 10 mu M AFB1 and 1 mu M DON significantly affected porcine oocyte maturation compared with 50 mu M AFB1 and 2 mu M DON on mouse oocytes. However, 10 nM HT-2 significantly affected mouse oocyte maturation compared with 50 nM HT-2 on porcine oocytes. Moreover, 5 mu M OTA and 10 mu M ZEA significantly affected porcine oocyte maturation compared with 300 mu M OTA and 50 mu M ZEA on mouse oocytes. In summary, our results showed that porcine oocytes were more sensitive to AFB1, DON, OTA, and ZEA than mouse oocytes except HT-2 toxin.
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