Journal
PEERJ
Volume 6, Issue -, Pages -Publisher
PEERJ INC
DOI: 10.7717/peerj.4292
Keywords
Burst swimming; Endurance test; Sustained swimming; Technique; Circular
Categories
Funding
- UNSW [FT130100268]
- ARC Discovery Early Career Research Award [DE150101774]
- UNSW VC Research Fellowship
- NHMRC Project Grants [GNT1063981, GNT1130222]
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Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34-0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes.
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