Journal
PEERJ
Volume 6, Issue -, Pages -Publisher
PEERJ INC
DOI: 10.7717/peerj.5086
Keywords
Geobacillus stearothermophilus; Biotransformation; Starch; Chisomaltooligosaccharide; Fermentation; Isomaltooligosaccharide
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Funding
- Technology Program of Gansu Province [1604FKCA110, 1606RJZA087, 1610RJZA060]
- Fundamental Research Funds for the Central Universities of China [lzujbky-2017-197]
- Project of Lanzhou City for Innovative and Entrepreneurial Talents [2017-RC73]
- Science and Technology Project of Lanzhou City [2015-3-93, 2016-3-75, 2017-4-122]
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The present study first identified the biotransformation of starch as a novel preparation method was investigated using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2. Subsequently, 5 L- and 20 L-scale fermentations were performed. After isolation and purification, liquid alpha-glucosidase preparations were obtained. Through covalent cross-linking and adsorption cross-linking using chitosan as the carrier and glutaraldehyde as the crosslinking agent, the conditions for immobilization of alpha-glucosidase on chitosan were determined. Moreover, Isomaltooligosaccharides (IMOs) were then prepared using chitosan membrane-immobilized alpha-glucosidase, beta-amylase, pullulanase, fungal alpha-amylase and starch as substrate. The mixed syrup that contained IMOs was evaluated and analyzed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). In addition, small-scale preparation of IMOs was performed. These results are a strong indication that the alpha-transglucosidase-producing G. stearothermophilus as a potential application technique can be successfully used to prepare industrial IMOs.
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