Journal
MOLECULAR THERAPY-NUCLEIC ACIDS
Volume 11, Issue -, Pages 272-283Publisher
CELL PRESS
DOI: 10.1016/j.omtn.2018.02.012
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Funding
- National Natural Science Foundation of China [31772574]
- Program of National Beef Cattle and Yak Industrial Technology System [CARS-37]
- Applied Basic Research Program of Qinghai Province [2014-ZJ-710]
- Special Fund of Xinyang Normal University [2017001]
- bio-breeding capacity-building and industry specific projects from National Development and Reform Commission [2014-2573]
- Specific Projects of Science and Technology in Henan Province [141100110200]
- Science and Technology Co-ordinator Innovative Engineering projects of Shaanxi Province [2015KTCL02-08]
- project of breeding and application of Pinan Cattle
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Muscle development is regulated under a series of complicate processes, and non-coding RNAs, such as microRNAs (miRNAs) and circular RNAs (circRNAs), have been reported to play important roles in regulating myoblast proliferation and differentiation. We found that miR-107 expression was high in skeletal muscle of Qinchuan cattle. Overexpression of miR-107 inhibited bovine myoblasts differentiation and protected cells from apoptosis. Wnt3a was identified as a target of miR-107 by luciferase activity, real-time qPCR, and western blotting assays. Knockdown of Wnt3a inhibited bovine myoblasts differentiation and apoptosis, and this effect was similar to miR-107 overexpression. We also found circFGFR4 to promote myoblasts differentiation and to induce cell apoptosis. Via luciferase screening and RNA pull-down assays, circFGFR4 was observed to sponge miR-107. Overexpression of circFGFR4 increased the expression of Wnt3a, whereas this effect was abolished by miR-107. These results demonstrated that circFGFR4 binding miR-107 promotes cell differentiation via targeting Wnt3a in bovine primary myoblasts.
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