Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 7, Issue 11, Pages 6238-6243Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b01508
Keywords
microPNA; tetrahedral DNA nanostructure; strand displacement reaction; silver nanoparticles; linear sweep voltammetry
Funding
- National Natural Science Foundation of China [31400847]
- Natural Science Foundation of Jiangsu Province of China [BK20141204]
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MicroRNAs are not only important regulators of a wide range of cellular processes but are also identified as promising disease biomarkers. Due to the low contents in serum, microRNAs are always difficult to detect accurately . In this study, an electrochemical biosensor for ultrasensitive detection of microRNA based on tetrahedral DNA nanostructure is developed. Four DNA single strands are engineered to form a tetrahedral nanostructure with a pendant stem-loop and modified on a gold electrode surface, which largely enhances the molecular recognition efficiency. Moreover, taking advantage of strand displacement polymerization, catalytic recycling of microRNA, and silver nanoparticle-based solid-state Ag/AgCl reaction, the proposed biosensor exhibits high sensitivity with the limit of detection down to 0.4 fM. This biosensor shows great clinical value and may have practical utility in early diagnosis and prognosis of certain diseases.
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