Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 131, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/56785
Keywords
Immunology; Issue 131; NK cell; feeder-free; gene silencing; TGF-beta 1; differentiation; OP9
Categories
Funding
- Research Grants Council of Hong Kong [GRF 468513, CUHK3/CRF/12R]
- Innovation and Technology Fund of Hong Kong [ITS/227/15, ITS InP/164/16, ITS-InP/242/16]
- Direct Grant for Research-CUHK [2016.035]
- Hong Kong Scholar Program
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Natural killer (NK) cells belong to the innate immune system and are a first-line anti-cancer immune defense; however, they are suppressed in the tumor microenvironment and the underlying mechanism is still largely unknown. The lack of a consistent and reliable source of NK cells limits the research progress of NK cell immunity. Here, we report an in vitro system that can provide high quality and quantity of bone marrow-derived murine NK cells under a feeder-free condition. More importantly, we also demonstrate that siRNA-mediated gene silencing successfully inhibits the E4bp4-dependent NK cell maturation by using this system. Thus, this novel in vitro NK cell differentiating system is a biomaterial solution for immunity research.
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