Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 135, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/57543
Keywords
Neuroscience; Issue 135; oligodendrocytes; in vitro; primary cells; mouse; neuroscience; oligodendrocyte purification; immunomagnetic isolation
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Funding
- National Multiple Sclerosis Society [RG4591A1/2]
- National Institutes of Health [R03NS06740402]
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The efficient and robust isolation and culture of primary oligodendrocytes (OLs) is a valuable tool for the in vitro study of the development of oligodendroglia as well as the biology of demyelinating diseases such as multiple sclerosis and Pelizaeus-Merzbacher-like disease (PMLD). Here, we present a simple and efficient selection method for the immunomagnetic isolation of stage three O4(+) preoligodendrocytes cells from neonatal mice pups. Since immature OL constitute more than 80% of the rodent-brain white matter at postnatal day 7 (P7) this isolation method not only ensures high cellular yield, but also the specific isolation of OLs already committed to the oligodendroglial lineage, decreasing the possibility of isolating contaminating cells such as astrocytes and other cells from the mouse brain. This method is a modification of the techniques reported previously, and provides oligodendrocyte preparation purity above 80% in about 4 h.
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