Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 134, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/57367
Keywords
Bioengineering; Issue 134; Pyrrole; Electrical Stimulation; Neural Progenitor Cell; Conductive Polymer; Stroke; Stem Cell
Categories
Funding
- National Institutes of Health [K08NS098876]
- Stanford School of Medicine Dean's Postdoctoral Fellowship
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [K08NS089976] Funding Source: NIH RePORTER
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Stem cell therapy has emerged as an exciting stroke therapeutic, but the optimal delivery method remains unclear. While the technique of microinjection has been used for decades to deliver stem cells in stroke models, this technique is limited by the lack of ability to manipulate the stem cells prior to injection. This paper details a method of using an electrically conductive polymer scaffold for stem cell delivery. Electrical stimulation of stem cells using a conductive polymer scaffold alters the stem cell's genes involved in cell survival, inflammatory response, and synaptic remodeling. After electrical preconditioning, the stem cells on the scaffold are transplanted intracranially in a distal middle cerebral artery occlusion rat model. This protocol describes a powerful technique to manipulate stem cells via a conductive polymer scaffold and creates a new tool to further develop stem cell-based therapy.
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