Immune Biosensor Based on Silica Nanotube Hydrogels for Rapid Biochemical Diagnostics of Bovine Retroviral Leukemia

The presented paper is devoted to investigation of properties of silica nanofibers hydrogels for biosensors applications. SiO2 nanofibers were prepared by the electrospinning method from polymeric solutions, containing SiO2 precursor, followed by a calcination procedure to obtain highly crystalline, pure inorganic nanofiber materials. The structural properties have been investigated by Scanning electron microscopy (SEM). Hydrogels were prepared by mixing of SiO2 nanofibers in distilled water. Photoluminescence (PL) spectra (excitation with lambda=266 nm) of SiO2 hydrogels with immobilized antigens (Ag) and antibodies (Ab) in the range were studied in the region 370-620 nm. Experimental dependence of PL properties of SiO2 hydrogels with immobilized Ag on Ab concentration was studied. (C) 2011 Published by Elsevier Ltd.