Journal
PHYSICA STATUS SOLIDI C: CURRENT TOPICS IN SOLID STATE PHYSICS, VOL 8, NO 6
Volume 8, Issue 6, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pssc.201000155
Keywords
porous silicon microcavities; matrix metalloproteinase; AFM; SEM; FT-IR spectroscopy
Funding
- Ecos-North French- Mexican program [M06P01]
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Porous silicon microcavity (PSiMc) structures were used as support material for specific sensing of matrix metalloproteinases (MMPs). For lower concentrations of MMP-8, the structures were tested with two types of functionalization methods. Silanization of the oxidized porous silicon structures, followed by glutaraldehyde chemistry was found to give very inconsistent results. The use of biotinilated bovin serum albumin linked to the naked PSiMc was found to be an alternative method to attach the anti MMP-8 human antibody, previously modified with streptavidin, which was further used to sense MMP-8. (C) 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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