4.3 Article

Efficient CRISPR-based genome editing using tandem guide RNAs and editable surrogate reporters

Journal

FEBS OPEN BIO
Volume 8, Issue 7, Pages 1167-1175

Publisher

WILEY
DOI: 10.1002/2211-5463.12437

Keywords

all-in-one; CRISPR/Cas9; gene-editing efficiency; surrogate reporter; tandem sgRNAs

Funding

  1. National Basic Research Program of China (973 Program) [2014CB943102]

Ask authors/readers for more resources

Cleavage efficiency plays a key role in clustered regularly interspaced short palindromic repeat (CRISPR)-based gene editing, particularly when the given guide RNA exhibits low cleavage activity. Here, we describe the packaging of tandem guide RNAs and single-strand annealing-based surrogate reporter cassettes into the CRISPR/CRISPR-associated protein 9 vector, which increased gene-editing efficiency by 4.94-6.31-fold and simultaneously enriched the proportion of genetically modified cells. This strategy may substantially improve genome-editing efficiency for demanding applications.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.3
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available