Journal
FEBS OPEN BIO
Volume 8, Issue 7, Pages 1167-1175Publisher
WILEY
DOI: 10.1002/2211-5463.12437
Keywords
all-in-one; CRISPR/Cas9; gene-editing efficiency; surrogate reporter; tandem sgRNAs
Categories
Funding
- National Basic Research Program of China (973 Program) [2014CB943102]
Ask authors/readers for more resources
Cleavage efficiency plays a key role in clustered regularly interspaced short palindromic repeat (CRISPR)-based gene editing, particularly when the given guide RNA exhibits low cleavage activity. Here, we describe the packaging of tandem guide RNAs and single-strand annealing-based surrogate reporter cassettes into the CRISPR/CRISPR-associated protein 9 vector, which increased gene-editing efficiency by 4.94-6.31-fold and simultaneously enriched the proportion of genetically modified cells. This strategy may substantially improve genome-editing efficiency for demanding applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available