Journal
TRANSPLANTATION RESEARCH
Volume 1, Issue -, Pages -Publisher
BMC
DOI: 10.1186/2047-1440-1-6
Keywords
Liver; Transplantation; Rat; Machine; Perfusion; Preservation; Ischemia; Donor; Shortage
Categories
Funding
- National Institutes of Health [R01DK096075, R01EB008678, R00DK080942]
- Shriners Hospitals for Children
- Health Resources and Services Administration contract [234-2005-37011C]
- Michael-van Vloten Foundation
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Background: Liver donor shortages stimulate the development of strategies that incorporate damaged organs into the donor pool. Herein we present a simplified machine perfusion system without the need for oxygen carriers or temperature control, which we validated in a model of orthotopic liver transplantation. Methods: Rat livers were procured and subnormothermically perfused with supplemented Williams E medium for 3 hours, then transplanted into healthy recipients (Fresh-SNMP group). Outcome was compared with static cold stored organs (UW-Control group). In addition, a rat liver model of donation after cardiac death was adapted using a 60-minute warm ischemic period, after which the grafts were either transplanted directly (WI group) or subnormothermically perfused and transplanted (WI-SNMP group). Results: One-month survival was 100% in the Fresh-SNMP and UW-Control groups, 83.3% in the WI-SNMP group and 0% in the WI group. Clinical parameters, postoperative blood work and histology did not differ significantly between survivors. Conclusion: This work demonstrates for the first time in an orthotopic transplantation model that ischemically damaged livers can be regenerated effectively using practical subnormothermic machine perfusion without oxygen carriers.
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