Journal
PLATELETS AND MEGAKARYOCYTES, VOL 3: ADDITIONAL PORTOCOLS AND PERSPECTIVES
Volume 788, Issue -, Pages 175-192Publisher
HUMANA PRESS INC
DOI: 10.1007/978-1-61779-307-3_13
Keywords
Bone marrow; Electron microscopy; Explants; In situ megakaryocyte ultrastructure; Proplatelet formation
Ask authors/readers for more resources
Differentiation and maturation of megakaryocytes occur in close association with cellular and extracellular components in the bone marrow. Thus, direct examination of these processes in the native environment provides important information regarding the development of megakaryocytes. In this chapter, we present methods applied to mouse bone marrow to (1) examine the ultrastructure of megakaryocytes and their state of maturation in situ in fixed bone marrow sections and (2) study the dynamics of proplatelet formation by real-time observation of fresh bone marrow explants where megakaryocytes have matured in their natural physiological context. Combining these two approaches allows detailed investigation of in situ megakaryocyte differentiation, including proplatelet formation, which is the final maturation step before platelet release.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available