Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 6, Issue 23, Pages 3922-3926Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8tb00414e
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Funding
- National Natural Science Foundation of China [21775084, 21621003, 21390410]
- China Postdoctoral Science Foundation [2016M601000]
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A novel fluorescent probe DNBS-CSA is developed for light-up detection of beta-lactamase. The probe design is based on an indirect detection approach with three step reactions. beta-Lactamase can react with the lactam of its substrate (cefazolin sodium) to produce a secondary amine, initiating a spontaneous elimination reaction and affording a thiol compound. The thiol could further react with the sulfonate group of DNBS-CSA, releasing the salicylaldehyde azine derivative (CSA) with both aggregation induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) characteristics. Previously reported beta-lactamase probes require covalent linkage of the substrate beta-lactam ring part to the probe, which makes probe synthesis difficult due to the complicated structure of the beta-lactam ring. In contrast, modification of the beta-lactam ring is no longer necessary for DNBS-CSA according to our indirect detection approach. The linear range of fluorescence quantification for beta-lactamase is 0-10 mU mL(-1) in an aqueous solution. Moreover, owing to the AIE properties of CSA, detection of beta-lactamase with DNBS-CSA on test papers was also achieved.
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