Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 6, Issue 18, Pages 2894-2900Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8tb00298c
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Funding
- NSFC [21472067, 21672083]
- Taishan Scholar Foundation [TS 201511041]
- University of Jinan [309-10004]
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Monitoring the intracellular viscosity changes is crucial for better understanding diffusion-controlled cellular processes. Herein, we synthesized a novel phenyl-substituted imidazole-fused rhodamine analogue RV-1 with a long absorption wavelength at 573 nm and a large Stokes shift of about 82 nm for monitoring the changes in cellular viscosity. The new probe RV-1 showed a very strong fluorescence emission at around 655 nm, with a 48.5-fold enhancement of fluorescence intensity from methanol to 99% glycerol. Significantly, the innovative probe RV-1 was successfully applied for the detection of viscosity changes not only in living cells, but also in zebra fish and living mice.
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