Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 6, Issue 15, Pages 2187-2197Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8tb00301g
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Funding
- California Institute for Regenerative Medicine [RT3-07899]
- National Institutes of Health [R01EB021857, R21HD090662]
- National Science Foundation [1547005, 1644967]
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1644967, 1547005] Funding Source: National Science Foundation
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Hydrogel scaffolds that mimic the native extracellular matrix (ECM) environment play a crucial role in tissue engineering. It has been demonstrated that cell behaviors can be affected by not only the hydrogel's physical and chemical properties, but also its three dimensional (3D) geometrical structures. In order to study the influence of 3D geometrical cues on cell behaviors as well as the maturation and function of engineered tissues, it is imperative to develop 3D fabrication techniques for creating micro and nanoscale hydrogel constructs. Among existing techniques that can effectively pattern hydrogels, two-photon polymerization (2PP)-based femtosecond laser 3D printing technology allows one to produce hydrogel structures with a resolution of 100 nm. This article reviews the basics of this technique and some of its applications in tissue engineering.
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