Journal
NUCLEAR MEDICINE AND MOLECULAR IMAGING
Volume 46, Issue 1, Pages 20-26Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13139-011-0109-5
Keywords
Porphyrins; Ga-68; Fibrosarcoma; Biodistribution; Co-incidence
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Aim Due to the interesting pharmacologic properties of porphyrins, the idea of developing a possible tumor imaging agent using PET by incorporating Ga-68 into a suitable porphyrin ligand was investigated. Methods 68Ga-labeled 5,10,15,20-tetrakis(pentafluoro-13 phenyl) porphyrin (Ga-68-TFPP) was prepared using freshly eluted [Ga-68] GaCl3 obtained from a Ge-68/Ga-68 generator developed in-house and 5,10,15,20-tetrakis(pentafluorophenyl) porphyrin (H2TFPP) for 60 min at 100 degrees C. Results The complex was prepared with high radiochemical purity (>99% ITLC, >99% HPLC, specific activity: 13-14 GBq/mmol). Stability of the complex was checked in the final formulation and in human serum for 5 h. The partition coefficient was calculated for the compound (log P = 0.62). The biodistribution of the labeled compound in vital organs of Swiss mice bearing fibrosarcoma tumors was studied using scarification studies and SPECT imaging up to 1 h. The complex was mostly washed out from the circulation through kidneys and liver. The tumor-to-muscle ratio 1 h post injection was 5.13. Conclusion The radiolabeled porphyrin complex demonstrated potential for further imaging studies in other tumor models.
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