Journal
CHINESE JOURNAL OF NATURAL MEDICINES
Volume 10, Issue 2, Pages 150-154Publisher
CHINESE JOURNAL NATURAL MEDICINES
DOI: 10.3724/SP.J.1009.2012.00150
Keywords
Adiponectin; Multimerization; Tanshinone IIA; AMPK; 3T3-L1 Adipocytes
Funding
- National Natural Science Foundation of China [30470354, 30570356]
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AIM: To investigate the effect of tanshinone HA on adiponectin biosynthesis. METHODS: The differentiation state of 3T3-L1 adipocytes was checked by Oil Red O staining. mRNA levels were examined by real-time PCR. Adiponectin oligomers were subjected to 2%-15% gradient SDS-PAGE and then detected using western blotting. Gene silencing experiments were performed using standard siRNA methods. RESULTS: It was found that tanshinone IIA, a diterpenoid naphthoquinone from the Chinese traditional herb Danshen (Salvia miltiorrhiza), inhibited the differentiation of preadipocytes and down-regulated the expression of adiponectin. In contrast, tanshinone IIA promoted the assembly of high-molecular weight (HMW) adiponectin during differentiation and in mature adipocytes. Tanshinone IIA activated AMP-activated protein kinase (AMPK) in 3T3-L1 adipocytes, and suppression of the AMPK pathway blocked the effect of tanshinone IIA on adiponectin expression and multimerization. CONCLUSION: These data suggest that tanshinone IIA promotes the assembly of HMW adiponectin through a process involving AMPK activation.
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