Journal
INTERDISCIPLINARY SCIENCES-COMPUTATIONAL LIFE SCIENCES
Volume 10, Issue 2, Pages 455-465Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s12539-018-0298-z
Keywords
CRISPR; CRISPR/Cas9; SgRNA design; On-target efficiency; Off-target detection
Categories
Funding
- National Key R&D Program of China [2017YFB0202602, 2017YFC1311003, 2016YFC1302500, 2016YFB0200400, 2017YFB0202104]
- NSFC [61772543, U1435222, 61625202, 61272056]
- Funds of State Key Laboratory of Chemo/Biosensing and Chemometrics
- Fundamental Research Funds for the Central Universities
- Guangdong Provincial Department of Science and Technology [2016B090918122]
Ask authors/readers for more resources
The adaptive immunity system in bacteria and archaea, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a powerful gene editing tool and got a broad application in genome research field due to its ease of use and cost-effectiveness. The performance of CRISPR/Cas relies on well-designed single-guide RNA (sgRNA), so a lot of bioinformatic tools have been developed to assist the design of highly active and specific sgRNA. These tools vary in design specifications, parameters, genomes and so on. To help researchers to choose their proper tools, we reviewed various sgRNA design tools, mainly focusing on their on-target efficiency prediction model and off-target detection algorithm.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available