Journal
GENETICS
Volume 209, Issue 4, Pages 1197-1224Publisher
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.118.300985
Keywords
meiotic entry; meiotic development; germline; PROM-1; SCF; GLD-1; GLD-2
Categories
Funding
- National Human Genome Research Institute
- National Institutes of Health Office of Research Infrastructure Programs [P40 OD-010440]
- Shohei Mitani and the Japanese National Bioresource Project
- National Institutes of Health [R01 GM-100756]
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Austrian Science Fund [SFB F3415-B19]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM100756] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [P40OD010440] Funding Source: NIH RePORTER
Ask authors/readers for more resources
A major event in germline development is the transition from stem/progenitor cells to entry into meiosis and gametogenesis. This transition requires downregulation of mitotic cell cycle activity and upregulation of processes associated with meiosis. We identify the Caenorhabditis elegans SCFPROM-1 E3 ubiquitin-ligase complex as functioning to downregulate mitotic cell cycle protein levels including cyclin E, WAPL-1, and KNL-2 at meiotic entry and, independently, promoting homologous chromosome pairing as a positive regulator of the CHK-2 kinase. SCFPROM-1 is thus a novel regulator of meiotic entry, coordinating downregulation of mitotic cell cycle proteins and promoting homolog pairing. We further show that SCFPROM-1 functions redundantly, in parallel to the previously described GLD-1 and GLD-2 meiotic entry pathways, downstream of and inhibited by GLP-1 Notch signaling, which specifies the stem cell fate. Accordingly, C. elegans employs three post-transcriptional pathways, SCFPROM-1-mediated protein degradation, GLD-1mediated translational repression, and GLD-2-mediated translational activation, to control and coordinate the initiation of meiotic development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available