Journal
GENE REGULATORY NETWORKS: METHODS AND PROTOCOLS
Volume 786, Issue -, Pages 181-200Publisher
HUMANA PRESS INC
DOI: 10.1007/978-1-61779-292-2_11
Keywords
Cap-analysis gene expression; RNAseq; Transcriptome; Sequencing; RNA
Funding
- NHGRI NIH HHS [U54 HG004557] Funding Source: Medline
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We provide here a protocol for the preparation of cap-analysis gene expression (CAGE) libraries, which allows for measuring the expression of eukaryotic capped RNAs and simultaneously map the promoter regions. The presented protocol simplifies the previously published ones and moreover produces tags that are 27 nucleotides long, which facilitates mapping to the genome. The protocol takes less than 5 days to complete and presents a notable improvement compared to previously published versions.
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