Journal
FRONTIERS IN MICROBIOLOGY
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2018.01555
Keywords
silver nanoparticles; tea leave extracts; antimicrobial activity; foodborne pathogens; Gram-negative; time-kill curves
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Funding
- Research University Grant Scheme Initiative Six (RUGS 6) of Universiti Putra Malaysia [GP-IPS 9438703]
- Fundamental Research Grant Scheme (FRGS) of Ministry of Higher Education (MOHE), Malaysia [02-01-14-1475FR]
- Kakenhi, Japan Society for the Promotion of Sciences [KAKENHI 24249038]
- Ministry of Health, Labor and Welfare, Japan
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Silver nanoparticles (AgNPs) used in this study were synthesized using pu-erh tea leaves extract with particle size of 4.06 nm. The antibacterial activity of green synthesized AgNPs against a diverse range of Gram-negative foodborne pathogens was determined using disk diffusion method, resazurin microtitre-plate assay (minimum inhibitory concentration, MIC), and minimum bactericidal concentration test (MBC). The MIC and MBC of AgNPs against Escherichia coli, Klebsiella pneumoniae, Salmonella Typhimurium, and Salmonella Enteritidis were 7.8, 3.9, 3.9, 3.9 and 7.8, 3.9, 7.8, 3.9 mu g/mL, respectively. Time-kill curves were used to evaluate the concentration between MIC and bactericidal activity of AgNPs at concentrations ranging from 0 x MIC to 8 x MIC. The killing activity of AgNPs was fast acting against all the Gram-negative bacteria tested; the reduction in the number of CFU mL(-1) was > 3 Log(10) units (99.9%) in 1-2 h. This study indicates that AgNPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agents for the treatment of bacterial infection including multidrug resistant bacterial infection.
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