Journal
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Volume 8, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2018.00220
Keywords
rabbits; NOD2; NF-kappa B; antibacterial ability; EHEC
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Funding
- Project of Natural Science Foundation of Shandong Province [ZR2017JL018]
- 13th five-year plan National Key Research and Development Program of China [2016YFD0500905]
- Key Research Project of Shandong Province [2016GNC110014]
- Shandong Double Tops Program [SYL2017YSTD11]
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Nucleotide-binding oligomerization domain 2 (NOD2), a member of the NOD-like receptors (NLRs) family that is well-known to play a key role in innate immune responses and is involved in innate antibacterial responses. In this study, rabbit NOD2 (rNOD2) was cloned from rabbit kidney (RK) cells. It was distributed in various tissues, and the highest level of rNod2 was detected in spleen. Moreover, the expression of rNod2 was significantly upregulated in the heart, liver, and spleen induced by enterohemorrhagic Escherichia coli (EHEC). Overexpression of rNOD2 induced the expression of pro-inflammatory cytokine, including Il1 beta,Il6, Ifn-gamma, and Tnf, as well as defensins, including Defb124,Defb125, and Defb128 through the nuclear factor (NF)-kappa B signaling pathway. Furthermore, overexpression of rNOD2 inhibited the growth of EHEC, and knockdown of rNOD2 or inhibition of the NE-kappa B pathway promoted its replication. In addition, our results suggest that rNOD2 can significantly activate NF-kappa B signaling and trigger antibacterial defenses to increase the expression of pro-inflammatory cytokine and defensins after stimulation by EHEC. These findings are useful to further understanding the innate immune system of rabbits and providing a new perspective for the prevention of bacterial diseases in rabbits.
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