Journal
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Volume 8, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2018.00155
Keywords
acyl-homoserine lactone; cattle rumen; E. coli; pig intestine; quorum sensing
Categories
Funding
- Natural Science Foundation of Jiangsu Province [BK20150442]
- 13th Five-Year National Key Development Program [2016YFD0501000, 2016YFD0500905]
- Chinese National Science Foundation Grants [31502075, 31672579, 31270171, 31072136, 30771603]
- Genetically Modified Organisms Technology Major Project of China [2014ZX08006-001B]
- Program for ChangJiang Scholars and Innovative Research Team In University PCSIRT [IRT0978]
- 948 programme from Ministry of Agriculture of the People's Republic of China [2011-G24]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Ask authors/readers for more resources
Quorum sensing systems regulate gene expression in response to bacterial population density. Acyl-homoserine lactones are a class of quorum sensing molecules found in cattle rumen that are thought to regulate the gene expression of enterohemorrhagic Escherichia coil and thus help this pathogen survive in animal gastrointestinal tracts. However, the specific bacteria that produce these signaling molecules in bovine and porcine gastrointestinal tracts are unknown. Here we developed methods to concentrate gastrointestinal fluids and screen the bacteria that produce acyl-homoserine lactones. We isolated a Pseudomonas aeruginosa strain YZ1 from cattle rumen, and an Aeromonas hydrophila strain YZ2 from pig intestine. Mass spectrometry analysis of culture supernatants indicated at least three specific classes of acyl-homoserine lactones produced by YZ1, and a C4-acyl-homoserine lactone produced by YZ2. Transformation of E coil with P aeruginosa or A. hydrophila luxi homologs,which can produce short- or long-chain acyl-homoserine lactones conferred upon E. coll. the ability to synthesize acyl-homoserine lactones and affected gene expression, motility, and acid tolerance of E. coll. This is the first study reporting the isolation and characterization of acyl-homoserine lactone synthase-positive bacteria from cattle rumen and swine intestines.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available