Journal
ELIFE
Volume 7, Issue -, Pages -Publisher
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.32692
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Funding
- Tulane University
- National Institute of Environmental Health Sciences [NIH R01ES028271, NIH R56ES026821, NIH R00 ES016780, NIH P20 RR020152]
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Tumors defective for DNA polymerase (Pol) epsilon proofreading have the highest tumor mutation burden identified. A major unanswered question is whether loss of Pol epsilon proofreading by itself is sufficient to drive this mutagenesis, or whether additional factors are necessary. To address this, we used a combination of next generation sequencing and in vitro biochemistry on human cell lines engineered to have defects in Pol epsilon proofreading and mismatch repair. Absent mismatch repair, monoallelic Pol epsilon proofreading deficiency caused a rapid increase in a unique mutation signature, similar to that observed in tumors from patients with biallelic mismatch repair deficiency and heterozygous Pol epsilon mutations. Restoring mismatch repair was sufficient to suppress the explosive mutation accumulation. These results strongly suggest that concomitant suppression of mismatch repair, a hallmark of colorectal and other aggressive cancers, is a critical force for driving the explosive mutagenesis seen in tumors expressing exonuclease-deficient Pol epsilon.
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