Journal
BIOTECHNOLOGY JOURNAL
Volume 13, Issue 5, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201700517
Keywords
alpha-Amylase; lactate dehydrogenase; lactate racemase; Lactobacillus plantarum; L-Lactic acid
Funding
- Japan Society for the Promotion of Sciences (JSPS), KAKENHI Grant [16K18299]
- Japan Science and Technology Agency (JST), MIRAI program
- Noda Institute for Scientific Research
- Grants-in-Aid for Scientific Research [16K18299] Funding Source: KAKEN
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Fermentative production of optically pure lactic acid (LA) has attracted great interest because of the increased demand for plant-based plastics. For cost-effective LA production, an engineered Lactobacillus plantarum NCIMB 8826 strain, which enables the production of optically pure l-LA from raw starch, is constructed. The wild-type strain produces a racemic mixture of d- and l-LA from pyruvate by the action of the respective lactate dehydrogenases (LDHs). Therefore, the gene encoding D-LDH (ldhD) is deleted. Although no decrease in d-LA formation is observed in the ldhD mutant, additional disruption of the operon encoding lactate racemase (larA-E), which catalyzes the interconversion between d- and l-LA, completely abolished d-LA production. From 100gL(-1) glucose, the ldhD larA-E mutant produces 87.0gL(-1) of l-LA with an optical purity of 99.4%. Subsequently, a plasmid is introduced into the ldhD larA-E mutant for the secretion of -aBmylase from Streptococcus bovis 148. The resulting strain could produce 50.3gL(-1) of l-LA from raw corn starch with a yield of 0.91 (g per g of consumed sugar) and an optical purity of 98.6%. The engineered L. plantarum strain would be useful in the production of l-LA from starchy materials.
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