Journal
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
Volume 46, Issue -, Pages 364-372Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/21691401.2018.1457537
Keywords
Calcium carbonate; nanoparticles; hyperthermia; radiotherapy; MCF-7 cells
Categories
Funding
- Solid Tumor Research Center of Urmia University of Medical Sciences (Urmia, Iran)
Ask authors/readers for more resources
The present study was undertaken to evaluate the synergistic effect of combining hyperthermia with irradiation and calcium carbonate nanoparticles (CC NPs) on proliferation of MCF-7 cells. The cells were randomly allocated to 19 groups: one negative control, three positive controls and 15 treatment groups. MCF-7 cells were treated with three concentrations of CC NPs (50, 100 and 150 mu g/mL), gamma radiation (200 cGy), hyperthermia (41 degrees C for 1 h) and three concentrations of doxorubicin (200, 400 and 800 nm) and incubated at 37 degrees C for 24h. Then the cell viability, the percentage of apoptosis and the levels of caspase-3, -8 and -9 proteins were measured. The results indicated that the combination group (150 mu g/mL CC NPs + thermoradiotherapy) had a significant (p < .001) decrease in cell viability (48.65 +/- 4.8%) and a significant (p < .001) increase in apoptosis percentage (45 +/- 1.63%) of MCF-7 cells, as compared with the negative control and most of the other treatment groups. Moreover, a significant (p < .05) increase was observed in the activity of caspase-3 and caspase-9. Our findings revealed that CC NPs in combination with irradiation and hyperthermia could significantly reduce the cell viability and enhance the apoptosis of the MCF-7 breast cancer cells, the same as doxorubicin anti-cancer drug.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available