Journal
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
Volume 33, Issue -, Pages 71-77Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.algal.2018.04.022
Keywords
Chlorella pyrenoidosa; Co-60-gamma irradiation mutagenesis; Lipid; Bioenergy
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Funding
- National Natural Science Foundation of China [31402319, 31372548]
- Key Scientific and Technological Project of Shan Xi Province [FT2014-01]
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In this study, the repeated mutagenic effects of Co-60-gamma irradiation combined with an optimized high-throughput screening method was applied to Chlorella pyrenoidosa to improve lipid productivity and characteristics for biodiesel. Two mutants of Chlorella pyrenoidosa, CP-232 and CP-269, were obtained. The lipid contents obtained from CP-232 and CP-269 were respectively increased by 20.8% and 20% under heterotrophic seed-photoautotrophic culture (HS-PC) model, and by 15% and 20.8% under photoautotrophic seed-photoautotrophic culture (PS-PC) model compared with the wild type strain in 1 L bubble column reactor. In addition, Co-60-gamma irradiation mutagenesis increased the proportion of saturated fatty acids in lipids accumulated in CP-232 and CP-269 compared to the wild type strain. In 3 L and 15 L bioreactor scale-up, lipid productivity was equally significantly increased in both CP-232 and CP-269 mutant strains. Our findings indicated that Co-60-gamma irradiation mutagenesis improves the accumulation of lipids with potential for industrial biodiesel production in mutant C. pyrenoidosa strains.
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