Journal
JOURNAL OF PHARMACEUTICAL ANALYSIS
Volume 2, Issue 2, Pages 152-155Publisher
ELSEVIER
DOI: 10.1016/j.jpha.2011.11.002
Keywords
Normal-phase HPTLC; Lamivudine; Zidovudine; Methanol
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Simultaneous quantification of Lamivudine and Zidovudine in tablets by HPTLC method was developed and validated. The chromatograms were developed using a mobile phase of toluene:ethyl acetate:methanol (4:4:2, v/v/v) on pre-coated plate of silica gel GF aluminum TLC plate and quantified by densitometric absorbance mode at 276 nm. The R-f values were 0.41 +/- 0.03 and 0.60 +/- 0.04 for Lamivudine and Zidovudine, respectively. The linearity of the method was found to be within the concentration range of 50-250 ng/spot for Lamivudine and for Zidovudine, it was 100-500 ng/spot. The lower limits of detection and quantification were 2.23 ng/spot and 7.90 ng/spot for Lamivudine and 2.90 ng/spot and 8.85 ng/spot for Zidovudine. The method was also validated for precision, specificity and recovery. This developed method was used to analyze fixed-dose tablets (Duovir, Cipla Ltd) samples of Lamivudine and Zidovudine. (C) 2011 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. All rights reserved.
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