Journal
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
Volume 6, Issue 4, Pages 4504-4509Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.8b00112
Keywords
Bacterial infections; Gold nanocluster; On-off-on fluorescent probe; Bacterial detection; Bactericide screening; Environmental monitoring
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Funding
- National Natural Science Foundation of China [31500802, 21628201]
- Natural Science Foundation of Jiangsu Province [BK20150350]
- Funds of Science Technology Department of Zhejiang Province [LGF18H180008]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- 333 Project of Jiangsu Province
- International Scientific Cooperation Project of Changzhou Scientific Bureau [CZ20160015]
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The bacterial differentiation, detection and bactericide screening are critical for environmental monitoring and human health maintenance. In this work, we present a novel strategy for rapid Escherichia coli (E. coli) differentiation, detection and bactericide screening, by employing an on off on gold nanocluster (AuNC)-based fluorescent probe. We demonstrate that by hijacking their copper-binding and redox pathways to capture and reduce Cu2+, E. coli bacteria can selectively and efficiently recover the fluorescence of AuNC from Cu2+-caused quenching. Based on this on off on AuNC-based fluorescent probe, rapid differentiation and detection of E. coli bacteria in artificial contaminated water sample with trace concentration (around 100 CFU/mL) is successfully realized within 0.5 h, showing great potential for rapid point-of-care detection of pathogenic E. coli bacteria in monitoring and clinical bedside diagnosis. Furthermore, this on-off-on AuNC-based fluorescent probe can enable rapid and accurate bactericide screening test for multidrug-resistant E. coli in sepsis blood sample, which is greatly helpful to accelerate the pace of treatment and reduce the mortality of infectious diseases.
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