Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 7, Issue 16, Pages 8377-8392Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b01254
Keywords
affinity materials; development; phosphoprotein; phosphopeptide; enrichment
Funding
- National Natural Science Foundation of China (NNSFC) [21171161]
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Reversible protein phosphorylation is a key event in numerous biological processes. Mass spectrometry (MS) is the most powerful analysis tool in modern phosphoproteomics. However, the direct MS analysis of phosphorylated proteins/peptides is still a big challenge because of the low abundance and insufficient ionization Of phosphorylated proteins/peptides as well as the suppression effects of nontargets. Enrichment of phosphorylated proteins/peptides by affinity material's from complex biosamples is the most widely used strategy to enhance the MS detection. The demand of efficiently enriching phosphorylated proteins/peptides has spawned diverse affinity materials based on different enrichment principles (e.g., electronic attraction, chelating). In this review, we summarize the recent development of various affinity materials for phosphorylated proteins] peptides enrichment. We will highlight the design and fabrication of these affinity materials, discuss the enrichment mechanisms involved in different affinity materials, and suggest the future challenges and research directions in this field.
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