Journal
POLYMERS
Volume 10, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/polym10010054
Keywords
formulation techniques; glass bead technique; giant vesicles; hybrid vesicles; phospholipids
Categories
Funding
- Swiss National Science Foundation [PP00P2_166209]
- COST Action [CM1304]
- Swiss National Science Foundation (SNF) [PP00P2_166209] Funding Source: Swiss National Science Foundation (SNF)
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Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles.
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