Journal
PLOS NEGLECTED TROPICAL DISEASES
Volume 12, Issue 4, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pntd.0006399
Keywords
-
Categories
Funding
- icddr,b
- Intramural Research Program of the National Institutes of Health, NIDDK
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases [R01 AI106878, AI103055, U01 AI058935, R03 AI063079, K08 AI089721, AI099243]
- Fogarty International Center, Training Grant in Vaccine Development and Public Health [D43 TW005572]
- Global Emerging Leader Award [K43 TW010362]
- Swedish Sida grant [INT-ICDDR,B-HN-01-AV]
- Physician Scientist Early Career Award from the Howard Hughes Medical Institute
- Robert Wood Johnson Foundation Harold Amos Medical Faculty Development Program
Ask authors/readers for more resources
Background The mediators of protection against cholera, a severe dehydrating illness of humans caused by Vibrio cholerae, are unknown. We have previously shown that plasma IgA as well as memory B IgG cells targeting lipopolysaccharide (LPS) of Vibrio cholerae O1 correlate with protection against V. cholerae O1 infection among household contacts of cholera patients. Protection against cholera is serogroup specific, and serogroup specificity is defined by the O-specific polysaccharide (OSP) component of LPS. Therefore, we prospectively followed household contacts of cholera patients to determine whether OSP-specific immune responses present at the time of enrollment are associated with protection against V. cholerae infection. Methodology In this study, we enrolled two hundred forty two household contacts of one hundred fifty index patients who were infected with Vibrio cholerae. We determined OSP-specific memory B cells and plasma IgA, IgG and IgM antibody responses on study entry (day 2). Principle findings The presence of OSP-specific plasma IgA, IgM, and IgG antibody responses on study entry were associated with a decrease in the risk of infection in household contacts (IgA, p = 0.015; IgM, p = 0.01, and IgG, p = 0.024). In addition, the presence of OSP-specific IgG memory B cell responses in peripheral blood on study entry was also associated with a decreased risk of infection (44% reduction; 95% CI: 31.1 to 99.8) in contacts. No protection was associated with cholera toxin B subunit (CtxB)-specific memory B cell responses. Conclusion These results suggest that immune responses that target OSP, both in plasma and memory responses, may be important in mediating protection against infection with V. cholerae O1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available