Journal
JOURNAL OF BIOMEDICAL SCIENCE
Volume 19, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1423-0127-19-9
Keywords
Reversine; cell cycle arrest; apoptosis; autophagy; oral squamous cell carcinoma (OSCC)
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Funding
- National Science Council [97-2311-B-194-001-MY3, NSC-99-2314-B-705-002-MY2]
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Background: The effective therapies for oral cancer patients of stage III and IV are generally surgical excision and radiation combined with adjuvant chemotherapy using 5-Fu and Cisplatin. However, the five-year survival rate is still less than 30% in Taiwan. Therefore, evaluation of effective drugs for oral cancer treatment is an important issue. Many studies indicated that aurora kinases (A, B and C) were potential targets for cancer therapies. Reversine was proved to be a novel aurora kinases inhibitor with lower toxicity recently. In this study, the potentiality for reversine as an anticancer agent in oral squamous cell carcinoma (OSCC) was evaluated. Methods: Effects of reversine on cell growth, cell cycle progress, apoptosis, and autophagy were evaluated mainly by cell counting, flow cytometry, immunoblot, and immunofluorescence. Results: The results demonstrated that reversine significantly suppressed the proliferation of two OSCC cell lines (OC2 and OCSL) and markedly rendered cell cycle arrest at G2/M stage. Reversine also induced cell death via both caspase-dependent and -independent apoptosis. In addition, reversine could inhibit Akt/ mTORC1 signaling pathway, accounting for its ability to induce autophagy. Conclusions: Taken together, reversine suppresses growth of OSCC via multiple mechanisms, which may be a unique advantage for developing novel therapeutic regimens for treatment of oral cancer in the future.
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