Journal
INTERNATIONAL JOURNAL OF POLYMER SCIENCE
Volume 2018, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2018/5790526
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Funding
- The Royal Golden Jubilee Ph.D. Program
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Dextran from Weissella confusa R003 isolated from sugar cane juice was purified and characterized. Dextran synthesis was performed by fermenting W. confusa R003 in MRS medium containing 10% (w/v) sucrose with continuous shaking at 125 rpm and at 30 degrees C. For 24 hours, the 50% efficiency yield was obtained. Dextran in the culture medium was purified by ethanol precipitation. Structural analysis of dextran using H-1 NMR, C-13 NMR, and 2D NMR techniques showed the existence of glucoses with 97.4% alpha (1 -> 6) linkage in the main chains and 2.6% alpha (1 -> 3) in branches. The estimation of molecular weight by dynamic light scattering exhibited average molecular weight of 1.0 x 10(4) kDa. At low concentration (2.5% w/v), dextran behaved like liquid structure, while, increasing the concentration (5.0 and 10.0% w/v), it was revealed as viscoelastic behavior. The highest gelling phenomenon was found in the concentration of 10% w/v and at 37 degrees C. Due to its production and properties, it may be suitable for commercial production and application in the field of foods as well as hydrogel.
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