Daphnia diversity on the Tibetan Plateau measured by DNA taxonomy

Daphnia on the Tibetan Plateau has been little studied, and information on species diversity and biogeography is lacking. Here, we conducted a 4-year survey using the barcoding fragment of the mitochondrial COI gene to determine the distribution and diversity of Daphnia species found across the Plateau. Our results show that species richness is higher than previously thought, with total described and provisional species number doubling from 5 to 10. Six of the taxonomic units recovered by DNA taxonomy agreed well with morphology, but DNA barcoding distinguished three clades each for the D.longispina (D.galeata, D.dentifera, and D.longispina) and D.pulex (D.pulex, D. cf. tenebrosa, and D.pulicaria) complexes. The sequence divergence between congeneric species varied within a large range, from 9.25% to 30.71%. The endemic D.tibetana was the most common and widespread species, occurring in 12 hyposaline to mesosaline lakes. The lineage of D.longispina is the first confirmed occurrence in west Tibet.