Journal
CELL REPORTS
Volume 23, Issue 13, Pages 3878-3890Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2018.05.091
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Funding
- NIH [R01DA029718, R21DA042597]
- Fundamental Research Funds for the Central Universities of China
- NIH intramural programs
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The role of astrocytes in neurovascular coupling (NVC) is unclear. Here, we applied a multimodality imaging approach to concomitantly measure synchronized neuronal or astrocytic Ca2+ and hemodynamic changes in the mouse somatosensory cortex at rest and during sensory electrical stimulation. Strikingly, we found that low-frequency stimulation (0.3-1 Hz), which consistently evokes fast neuronal Ca2+ transients (6.0 +/- 2.7 ms latency) that always precede vascular responses, does not always elicit astrocytic Ca2+ transients (313 +/- 65 ms latency). However, the magnitude of the hemodynamic response is increased when astrocytic transients occur, suggesting a facilitatory role of astrocytes in NVC. High-frequency stimulation (5-10 Hz) consistently evokes a large, delayed astrocytic Ca2+ accumulation (3.48 +/- 0.09 s latency) that is temporarily associated with vasoconstriction, suggesting a role for astrocytes in resetting NVC. At rest, neuronal, but not astrocytic, Ca2+ fluctuations correlate with hemodynamic low-frequency oscillations. Taken together, these results support a role for astrocytes in modulating, but not triggering, NVC.
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