Journal
CELL REPORTS
Volume 23, Issue 11, Pages 3209-3222Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2018.05.028
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Funding
- Whitehall Foundation [20120844]
- Japan Foundation for Pediatric Research
- Naito Foundation
- NIH [R01NS085215, R01MH104341, P50MH096890]
- [15K06732]
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Homeostatic synaptic downscaling reduces neuronal excitability by modulating the number of postsynaptic receptors. Histone modifications and the subsequent chromatin remodeling play critical roles in activity-dependent gene expression. Histone modification codes are recognized by chromatin readers that affect gene expression by altering chromatin structure. We show that L3mbtl1 (lethal 3 malignant brain tumor-like 1), a polycomb chromatin reader, is downregulated by neuronal activity and is essential for synaptic response and downscaling. Genome-scale mapping of L3mbtl1 occupancies identified Ctnnb1 as a key gene downstream of L3mbtl1. Importantly, the occupancy of L3mbtl1 on the Ctnnb1 gene was regulated by neuronal activity. L3mbtl1 knockout neurons exhibited reduced Ctnnb1 expression. Partial knockdown of Ctnnb1 in wild-type neurons reduced excitatory synaptic transmission and abolished homeostatic downscaling, and transfecting Ctnnb1 in L3mbtl1 knockout neurons enhanced synaptic transmission and restored homeostatic down-scaling. These results highlight a role for L3mbtl1 in regulating homeostasis of synaptic efficacy.
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