Journal
CELL REPORTS
Volume 22, Issue 1, Pages 44-58Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2017.12.037
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Funding
- ERC [339953]
- Danish National Research Council
- Lundbeck Foundation
- Novo Nordisk Foundation
- NIH grant [P41 GM109824, P41 GM103314]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P41GM109824, P41GM103314] Funding Source: NIH RePORTER
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Nuclear RNA metabolism is influenced by protein complexes connecting to both RNA-productive and -destructive pathways. The ZC3H18 protein binds the cap-binding complex (CBC), universally present on capped RNAs, while also associating with the nuclear exosome targeting (NEXT) complex, linking toRNAdecay. To dissect ZC3H18 function, we conducted interaction screening and mutagenesis of the protein, which revealed a phosphorylationdependent isoform. Surprisingly, the modified region of ZC3H18 associates with core histone proteins. Further examination of ZC3H18 function, by genome-wide analyses, demonstrated its impact on transcription of a subset of protein-coding genes. This activity requires the CBC-interacting domain of the protein, with some genes being also dependent on the NEXT-and/or histone-interacting domains. Our data shed light on the domain requirements of a protein positioned centrally in nuclear RNA metabolism, and they suggest that post-translational modification may modulate its function.
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