Journal
CELL REPORTS
Volume 24, Issue 3, Pages 685-700Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2018.06.071
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Funding
- Netherlands Organisation for Scientific Research [865.10.010]
- Netherlands Organisation for Health Research and Development [91215084]
- European Research Council (ERC) [617050]
- Deutsche Forschungsgemeinschaft [807223]
- European Research Council (ERC) [617050] Funding Source: European Research Council (ERC)
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Tight regulation of neuronal transport allows for cargo binding and release at specific cellular locations. The mechanisms by which motor proteins are loaded on vesicles and how cargoes are captured at appropriate sites remain unclear. To better understand how KIF1A-driven dense core vesicle (DCV) transport is regulated, we identified the KIF1A interactome and focused on three binding partners, the calcium binding protein calmodulin (CaM) and two synaptic scaffolding proteins: liprin-alpha and TANC2. We showed that calcium, acting via CaM, enhances KIF1A binding to DCVs and increases vesicle motility. In contrast, liprin-alpha and TANC2 are not part of the KIF1A-cargo complex but capture DCVs at dendritic spines. Furthermore, we found that specific TANC2 mutations-reported in patients with different neuropsychiatric disorders-abolish the interaction with KIF1A. We propose a model in which Ca2+/CaM regulates cargo binding and liprin-a and TANC2 recruit KIF1A-transported vesicles.
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