Journal
CELL REPORTS
Volume 23, Issue 8, Pages 2482-2494Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2018.04.072
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Funding
- NIH Office of Research Infrastructure Programs [P40 OD010440]
- Chinese Ministry of Science and Technology [2017YFA0102903]
- National Natural Science Foundation of China [81501329, 31671346, 91640110]
- Anhui Natural Science Foundation [1608085MC50]
- Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology [2017FXZY005]
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RNAi-elicited gene silencing is heritable and can persist for multiple generations after its initial induction in C. elegans. However, the mechanism by which parental-acquired trait-specific information from RNAi is inherited by the progenies is not fully understood. Here, we identified a cytoplasmic Argonaute protein, WAGO-4, necessary for the inheritance of RNAi. WAGO-4 exhibits asymmetrical translocation to the germline during early embryogenesis, accumulates at the perinuclear foci in the germline, and is required for the inheritance of exogenous RNAi targeting both germline- and soma-expressed genes. WAGO-4 binds to 22G-RNAs and their mRNA targets. Interestingly, WAGO-4-associated endogenous 22G-RNAs target the same cohort of germline genes as CSR-1 and contain untemplated addition of uracil at the 3' ends. The poly(U) polymerase CDE-1 is required for the untemplated uridylation of 22G-RNAs and inheritance of RNAi. Therefore, we conclude that, in addition to the nuclear RNAi pathway, the cytoplasmic RNAi machinery also promotes RNAi inheritance.
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