Journal
THERANOSTICS
Volume 8, Issue 6, Pages 1624-1635Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.23531
Keywords
circulating tumor cells; cell isolation; heterogeneous cells; precision medicine
Categories
Funding
- National Natural Science Foundation [81527801]
- National Natural Science Foundation of China [81371897]
- China Postdoctoral Science Foundation [2016M602367, 2017T100571]
- National Key Research and Development Project [2016YFC1000700]
- Hubei Province's Outstanding Medical Academic Leader Program
- Young One Thousand Talent program of China
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Background: Circulating tumor cells (CTCs) are a burgeoning topic in cancer biomarker discovery research with minimal invasive blood draws. CTCs can be used as potential biomarkers for disease prognosis, early cancer diagnosis and pharmacodynamics. However, the extremely low abundance of CTCs limits their clinical utility because of technical challenges such as the isolation and subsequent detailed molecular and functional characterization of rare CTCs from patient blood samples. Methods: In this study, we present a novel density gradient centrifugation method employing biodegradable gelatin nanoparticles coated on silicon beads for the isolation, release, and downstream analysis of CTCs from colorectal and breast cancer patients. Results: Using clinical patient/spiked samples, we demonstrate that this method has significant CTC-capture efficiency (> 80%) and purity (> 85%), high CTC release efficiency (94%) and viability (92.5%). We also demonstrate the unparalleled robustness of our method in downstream CTC analyses such as the detection of PIK3CA mutations. Conclusion: The efficiency and versatility of the multifunctional density microbeads approach provides new opportunities for personalized cancer diagnostics and treatments.
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