Journal
THERANOSTICS
Volume 8, Issue 5, Pages 1411-1420Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.22252
Keywords
fluorescence probe; GSH; Sepsis; cell imaging; diagnosis
Categories
Funding
- National Creative Research Initiative Program of the National Research Foundation of Korea (NRF) - Korean government (MSIP) [2012R1A3A2048814]
- Bio & Medical Technology Development Program of the NRF - Korean government, MSIP [2017M3A9F3041229]
- National Natural Science Foundation of China [21472118, 21672131]
- National Leading Research Lab Program of the NRF - MSIP [2016R1E1A1A02920873]
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Rationale: Among the biothiols-related diseases, sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection and can result in severe oxidative stress and damage to multiple organs. In this study, we aimed to develop a fluorescence chemosensor that can both detect GSH and further predict sepsis. Methods: In this study, two new naphthalene dialdehyde compounds containing different functional groups were synthesized, and the sensing abilities of these compounds towards biothiols and its applications for prediction of sepsis were investigated. Results: Our study revealed that the newly developed probe 6-methoxynaphthalene-2, 3-dicarbaldehyde (MNDA) has two-photon is capable of detecting GSH in live cells with two-photon microscopy (TPM) under the excitation at a wavelength of 900 nm. Furthermore, two GSH detection probes naphthalene-2,3-dicarboxaldehyde (NDA) and 6-fluoronaphthalene-2,3-dicarbaldehyde (FNDA) not only can detect GSH in living cells, but also showed clinical significance for the diagnosis and prediction of mortality in patients with sepsis. Conclusions: These results open up a promising direction for further medical diagnostic techniques.
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