Journal
THERANOSTICS
Volume 8, Issue 13, Pages 3461-3473Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.25179
Keywords
nanohybrid; nanoquencher; fluorescence; no-wash biosensor; ratiometric; cancer biomarker
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Funding
- National Natural Science Foundation of China [31760485, 21335004]
- Major Projects of Natural Science Foundation of Jiangxi province [20161ACB20002]
- Intramural Research Program, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
- China Scholarship Council (CSC) [201606820007, 201606190118]
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Purpose: Early diagnosis of cancer enables extended survival and reduced symptoms. To this end, a three-in-one nanohybrid of MOF@AuNP@GO is designed as synergistic nanoquencher to develop a novel fluorescence biosensor for rapid and sensitive detection of cancer-related biomarkers. Methods: The ssDNA absorption affinities and fluorescence quenching abilities of the MOF@AuNP@GO were evaluated using FAM-labeled single-stranded DNA (ssDNA). Then, two specific dye-labeled ssDNA and aptamer probes were designed for the recognition of p53 gene and prostate specific antigen (PSA), respectively. Fluorescence spectra were recorded and ratiometric signal processing was performed. Results: The designed nanohybrids exhibit enhanced ssDNA binding affinities and fluorescence quenching abilities, which significantly decrease the background signal and increase the signal-to-noise (S/N) ratio, thus lowering the detection limit (LOD). Accordingly, with ratiometric measurement, this developed nanosensor can sensitively measure p53 gene and PSA with LODs of 0.005 nM and 0.01 ng mL(-1), respectively. Besides, this method also displays excellent performances with respect to universality, multiplexed detection, specificity, and practicality in human serum. Conclusion: The designed MOF@AuNP@GO-based fluorescence biosensor can serve as a promising platform for washing-free, rapid and sensitive measurement of cancer biomarkers, making this method well-suited for point-of-care (POC) diagnosis.
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