Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection

Background: Induced pluripotent stem cells (iPS) represent an innovative source for the standardized in vitro generation of macrophages (M phi). Mf show great promise in disease pathogenesis, particularly tuberculosis. However, there is no information about human iPS-derived (hiPS) macrophages (hiPS-M phi) in response to tuberculosis infection. Methods: In the present study, macrophages derived from hiPS were established via embryoid body (EB) formation by using feeder-free culture conditions, and the human monocyte cell line THP-1 (THP-1-M phi) was used as control. iPS-M phi were characterized by using morphology, Giemsa staining, nonspecific esterase staining (alpha-NAE), phagocytosis, and surface phenotype. Additionally, after treatment with Bacillus Calmette-Guerin (BCG) for 24 h, cell apoptosis was detected by using an Annexin V-FITC Apoptosis Detection assay. The production of nitric oxide (NO), expression of tumor necrosis factor alpha (TNF-alpha), activity of apoptosis-related protein cysteine-3 (Caspase-3) and expression of B-cell lymphoma-2 (Bcl-2) were analyzed. Results: With respect to morphology, surface phenotype, and function, the iPS-M phi closely resembled their counterparts generated in vitro from a human monocyte cell line. iPS-M phi exhibited the typically morphological characteristics of macrophages, such as round, oval, fusiform and irregular characteristics. The cells were Giemsa-stained-positive, a-NAE-positive, and possessed phagocytic ability. iPS-M phi express high levels of CD14, CD11b, CD40, CD68, and major histocompatibility complex II(MHC-II). Moreover, with regard to the apoptotic rate, the production of NO, expression of TNF-alpha, and activity of Caspase-3 and Bcl-2, iPS-M phi closely resemble that of their counterparts generated in vitro from human monocyte cell line in response to BCG infection. The rate of apoptosis of BCG-treated iPS-M phi was 37.77 +/- 7.94% compared to that of the untreated group at 4.97 +/- 1.60% (P < 0.01) by using Annexin V-FITC Apoptosis Detection. Additionally, the rate of apoptosis of BCG-treated THP-1-M phi was 37.1 +/- 2.84% compared to that of the untreated group at 6.19 +/- 1.68% (P < 0.001). The expression of TNF-a and the production of NO were significantly increased (P < 0.001), and the activity of Caspase-3 was increased. However, the expression of Bcl-2 was inhibited (P < 0.001). Conclusions: Our results demonstrate that M phi derived from hiPS perform the immunological function in response to Bacillus Calmette-Guerin infection by undergoing apoptosis, increasing the production of NO and expression of TNF-a. Thus, our study may help to overcome the limitations of research into certain rare diseases due to the lack of adequate supply of disease-specific primary cells.