Journal
SCIENTIFIC REPORTS
Volume 8, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41598-018-27037-5
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Funding
- Renfrew Scholarship at the University of Idaho
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Hydrogel microparticles were copolymerized with surface-immobilized DNA. Particles derived from a microfluidic device and particles derived from mechanical homogenization were compared. The hypothesis was tested that a controlled droplet generation mechanism would produce more homogeneous particles. Surprisingly, the DNA content of both particle types was similarly inhomogeneous. To make this test possible, a simple, low cost, and rapid method was developed to fabricate a microfluidic chip for droplet generation and in-line polymerization. This method used a lowcost laser cutter ($400) and direct heat bonding (no adhesives or intermediate layers). The flow focusing droplet generator produced droplets and hydrogel particles 10-200 mu m in diameter.
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